Dissecting and Culturing Animal Cap Explants
نویسندگان
چکیده
منابع مشابه
xCITED2 Induces Neural Genes in Animal Cap Explants of Xenopus Embryos
Neural tissue is arisen from presumptive ectoderm via inhibition of bone morphogenetic protein (BMP) signaling during Xenopus early development. Previous studies demonstrate that ectopic expression of dominant negative BMP4 receptor (DNBR) produces neural tissue in animal cap explants (AC) and also increases the expression level of various genes involved in neurogenesis. To investigate detail m...
متن کاملDynamin-dependent endocytosis is necessary for convergent-extension movements in Xenopus animal cap explants.
Cadherin cell-cell adhesion molecules are important determinants of morphogenesis and tissue patterning. C-cadherin plays a key role in the cell-upon-cell movements seen during Xenopus gastrulation. In particular, regulated changes in C-cadherin adhesion critically influence convergence-extension movements, thereby determining organization of the body plan. It is also predicted that remodelling...
متن کاملArtefactual gene induction during preparation of Xenopus laevis animal cap explants.
The animal cap assay in Xenopus laevis was used to study the induction and regulation of the mesoderm-specific gene Xegr-1, a homolog of the mammalian egr-1 genes. Egr-1 is an immediate-early gene whose growth factor-stimulated transcriptional induction displays a transient activity profile and occurs independent of protein synthesis. The Xegr-1 promoter contains multiple serum response element...
متن کاملCulturing spinal cord explants in a collagen gel.
A new method for culturing spinal cord slices or explants is presented which entails the use of a commercially available purified collagen, Vitrogen. Vitrogen provides a stable three-dimensional matrix for culturing spinal cord explants which is superior to the conventional method of applying explants to moist dishes coated with rat tail collagen. The use of Vitrogen facilitated the culturing o...
متن کاملCulturing bovine nucleus pulposus explants by balancing medium osmolarity.
Regenerative therapies are promising treatments for early intervertebral disc degeneration. To test their efficacy, an in vitro tissue-level model would be valuable. Nucleus pulposus (NP) explant culture may constitute such a model, as the earliest signs of degeneration are in the NP. However, in NP explant cultures, balancing tissue osmolarity is crucial to preventing swelling, proteoglycan (P...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Cold Spring Harbor Protocols
سال: 2018
ISSN: 1940-3402,1559-6095
DOI: 10.1101/pdb.prot097329